Comparison of fixed and living biological cells parameters investigated with digital holographic microscope

Authors

  • Patryk Nienałtowski Institute of Micromechanics and Photonics Warsaw University of Technology
  • Maria Baczewska Institute of Micromechanics and Photonics Warsaw University of Technology
  • Małgorzata Kujawińska Institute of Micromechanics and Photonics Warsaw University of Technology

DOI:

https://doi.org/10.4302/plp.v12i1.971

Abstract

The statistical analysis and comparison of biophysical parameters of living and fixed, mouse embryonic fibroblasts cells are presented. The parameters are calculated based on phase measurements performed by means of a digital, holographic microscope. The phases are retrieved from off-axis, image plane holograms, followed by custom image segmentation and statistical analysis of cells’ surface, phase volume and dry mass. The results indicated statistically significant differences between fixed and living cell parameters, which is an important message for setting methodology for further diagnosis based on quantitative phase (label-free) analysis.

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References:

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Published

2020-03-31

How to Cite

[1]
P. Nienałtowski, M. Baczewska, and M. Kujawińska, “Comparison of fixed and living biological cells parameters investigated with digital holographic microscope”, Photonics Lett. Pol., vol. 12, no. 1, pp. 13–15, Mar. 2020.

Issue

Vol. 12 No. 1 (2020)

Section

Articles

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