Multiphoton scanning laser microscope based on femtosecond fiber laser

Authors

  • Alicja Kwaśny Laser and Fiber Electronics Group, Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, Wrocław, Poland
  • Jakub Bogusławski Laser and Fiber Electronics Group, Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, Wrocław, Poland
  • Grzegorz Soboń Laser and Fiber Electronics Group, Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, Wrocław, Poland

DOI:

https://doi.org/10.4302/plp.v14i4.1182

Keywords:

fiber lasers, multiphoton microscopy, biophotonics,

Abstract

We present a multiphoton scanning laser microscope based on a femtosecond frequency-doubled erbium-doped fiber laser. The laser used in the epi-illumination microscope setup generated 95 fs pulses at the wavelength of 780 nm with 44.3 mW average power at 100 MHz pulse repetition rate. The imaging process was controlled by custom software developed in the NI LabVIEW environment. Detection of two-photon fluorescence was proven by acquiring a series of images from various biological samples.

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References
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Published

2022-12-31

How to Cite

[1]
A. Kwaśny, J. Bogusławski, and G. Soboń, “Multiphoton scanning laser microscope based on femtosecond fiber laser”, Photonics Lett. Pol., vol. 14, no. 4, pp. 74–76, Dec. 2022.

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